What precaution is essential when using a centrifuge?

• A. Balance the sample
• B. Set to maximum speed
• C. Open rotor
• D. Use dirty tubes

Answer: (A)

Keeping the centrifuge load balanced is essential. When the masses aren’t equal around the rotor, it wobbles and vibrates, which can damage bearings, crack rotors or tubes, cause leaks or aerosols, and create a safety hazard for anyone nearby. So, placing tubes so that the total weight and distribution are even on opposite sides of the rotor is the key precaution.

Other options don’t fit safety and proper operation: setting the centrifuge to maximum speed bypasses the designed limits and can trigger rotor failure or accidents; opening the lid or rotor while spinning is extremely dangerous and must not be done until the rotor is stopped; using dirty tubes can contaminate samples and also alter the weight distribution, increasing imbalance risks.